A platform-based design environment for synthetic biological systems
The Fifth Richard Tapia Celebration of Diversity in Computing Conference: Intellect, Initiatives, Insight, and Innovations
Automated design of assemblable, modular, synthetic chromosomes
PPAM'09 Proceedings of the 8th international conference on Parallel processing and applied mathematics: Part II
A language for biochemical systems: design and formal specification
Transactions on Computational Systems Biology XII
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Motivation: The sequence of artificial genetic constructs is composed of multiple functional fragments, or genetic parts, involved in different molecular steps of gene expression mechanisms. Biologists have deciphered structural rules that the design of genetic constructs needs to follow in order to ensure a successful completion of the gene expression process, but these rules have not been formalized, making it challenging for non-specialists to benefit from the recent progress in gene synthesis. Results: We show that context-free grammars (CFG) can formalize these design principles. This approach provides a path to organizing libraries of genetic parts according to their biological functions, which correspond to the syntactic categories of the CFG. It also provides a framework for the systematic design of new genetic constructs consistent with the design principles expressed in the CFG. Using parsing algorithms, this syntactic model enables the verification of existing constructs. We illustrate these possibilities by describing a CFG that generates the most common architectures of genetic constructs in Escherichia coli. Availability: A web site allows readers to experiment with the algorithms presented in this article: www.genocad.org Contact: peccoud@vt.edu Supplementary information: Sequences and models are available at Bioinformatics online.