Computer techniques in neuroanatomy
Computer techniques in neuroanatomy
Using Dynamic Programming for Solving Variational Problems in Vision
IEEE Transactions on Pattern Analysis and Machine Intelligence
Dynamic Programming for Detecting, Tracking, and Matching Deformable Contours
IEEE Transactions on Pattern Analysis and Machine Intelligence
An Unbiased Detector of Curvilinear Structures
IEEE Transactions on Pattern Analysis and Machine Intelligence
Digital Image Processing Methods
Digital Image Processing Methods
Digital Image Processing
Extracting Curvilinear Structures: A Differential Geometric Approach
ECCV '96 Proceedings of the 4th European Conference on Computer Vision-Volume I - Volume I
CVRMed-MRCAS '97 Proceedings of the First Joint Conference on Computer Vision, Virtual Reality and Robotics in Medicine and Medial Robotics and Computer-Assisted Surgery
Multiscale detection of curvilinear structures in 2-D and 3-D image data
ICCV '95 Proceedings of the Fifth International Conference on Computer Vision
IEEE Transactions on Information Technology in Biomedicine
IEEE Transactions on Information Technology in Biomedicine
Rapid automated three-dimensional tracing of neurons from confocal image stacks
IEEE Transactions on Information Technology in Biomedicine
Snakes, shapes, and gradient vector flow
IEEE Transactions on Image Processing
Journal of Visual Communication and Image Representation
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The morphological properties of axons, such as their branching patterns and oriented structures, are of great interest for biologists in the study of the synaptic connectivity of neurons. In these studies, researchers use triple immunofluorescent confocal microscopy to record morphological changes of neuronal processes. Three-dimensional (3D) microscopy image analysis is then required to extract morphological features of the neuronal structures. In this article, we propose a highly automated 3D centerline extraction tool to assist in this task. For this project, the most difficult part is that some axons are overlapping such that the boundaries distinguishing them are barely visible. Our approach combines a 3D dynamic programming (DP) technique and marker-controlled watershed algorithm to solve this problem. The approach consists of tracking and updating along the navigation directions of multiple axons simultaneously. The experimental results show that the proposed method can rapidly and accurately extract multiple axon centerlines and can handle complicated axon structures such as cross-over sections and overlapping objects.