Classification and Quantification Based on Image Analysis for Sperm Samples with Uncertain Damaged/Intact Cell Proportions

  • Authors:
  • Lidia Sánchez;Víctor González;Enrique Alegre;Rocío Alaiz

  • Affiliations:
  • Computing and Industrial Engineering School, University of León, León, Spain 24071;Computing and Industrial Engineering School, University of León, León, Spain 24071;Computing and Industrial Engineering School, University of León, León, Spain 24071;Computing and Industrial Engineering School, University of León, León, Spain 24071

  • Venue:
  • ICIAR '08 Proceedings of the 5th international conference on Image Analysis and Recognition
  • Year:
  • 2008

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Abstract

Classifying damaged-intact cells in a semen sample presents the peculiarity that the test class distribution is unknown. This paper studies under which design conditions the misclassification rate is minimum for the uncertainty region of interest (ratio of damaged cells lower than 20%) and (b) deals with quantifying the proportion of damaged/intact sperm cells in a given sample based on computer vision and supervised learning. We have applied a discrete wavelet transform to the spermatozoa head images and computed the mean and standard deviation (WSF) and four Haralick descriptors (WCF). Using a backpropagation neural network, the error rate averaged over distributions in the region of interest is 4.85% with WCF. The assessment of several quantification methods shows the conditions under which the Adjusted Count method leads to an overall mean absolute error of 3.2 and the Classify & Count method yields 2.4, both with WCF features. Deviations of this order are considered reasonable for this field.