Structural Matching of 2D Electrophoresis Gels using Graph Models

  • Authors:
  • Alexandre Noma;Alvaro Pardo;Roberto M. Cesar Jr

  • Affiliations:
  • -;-;-

  • Venue:
  • SIBGRAPI '08 Proceedings of the 2008 XXI Brazilian Symposium on Computer Graphics and Image Processing
  • Year:
  • 2008

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Abstract

2D electrophoresis is a well known method for protein separation which is extremely useful in the field of proteomics.Each spot in the image represents a protein accumulation and the goal is to perform a differential analysis between pairs of images to study changes in protein content.It is thus necessary to register two images by finding spot correspondences. Although it may seem a simple task, generally,the manual processing of this kind of images is very cumbersome. The complete task of individual spot matching and gel registration is a complex and time consuming process when strong variations between corresponding sets of spots are expected. Besides, because an one-to-one mapping is expected between the two images,missing spots there may exist on both images (i.e. spots without correspondence). In order to solve this problem, this paper proposes a new distance together with a correspondence estimation algorithm based on graph matching which takes into account the structural information between the detected spots. Each image is represented by a graph and the task is to find an isomorphism between subgraphs. Successful experimental results using real data are presented,including a comparative performance evaluation.