Structural identification of GMA models: algorithm and model comparison
Proceedings of the 8th International Conference on Computational Methods in Systems Biology
A parameter estimation approach for non-linear systems biology models using spline approximation
Proceedings of the First ACM International Conference on Bioinformatics and Computational Biology
Hybrid method for the analysis of time series gene expression data
Knowledge-Based Systems
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Motivation: At the center of computational systems biology are mathematical models that capture the dynamics of biological systems and offer novel insights. The bottleneck in the construction of these models is presently the identification of model parameters that make the model consistent with observed data. Dynamic flux estimation (DFE) is a novel methodological framework for estimating parameters for models of metabolic systems from time-series data. DFE consists of two distinct phases, an entirely model-free and assumption-free data analysis and a model-based mathematical characterization of process representations. The model-free phase reveals inconsistencies within the data, and between data and the alleged system topology, while the model-based phase allows quantitative diagnostics of whether—or to what degree—the assumed mathematical formulations are appropriate or in need of improvement. Hallmarks of DFE are the facility to: diagnose data and model consistency; circumvent undue compensation of errors; determine functional representations of fluxes uncontaminated by errors in other fluxes and pinpoint sources of remaining errors. Our results suggest that the proposed approach is more effective and robust than presently available methods for deriving metabolic models from time-series data. Its avoidance of error compensation among process descriptions promises significantly improved extrapolability toward new data or experimental conditions. Contact: eberhard.voit@bme.gatech.edu Supplementary information: Supplementary data are available at Bioinformatics online.