Segmentation of cDNA microarray images by kernel density estimation

Authors:
Tai-Been Chen;Henry Horng-Shing Lu;Yun-Shien Lee;Hsiu-Jen Lan
Affiliations:
Institute of Statistics, National Chiao Tung University, 1101 Ta Hsueh Road, Hsinchu 30010, Taiwan, ROC and Department of Medical Imaging and Radiological Sciences, I-Shou University, Taiwan, ROC;Institute of Statistics, National Chiao Tung University, 1101 Ta Hsueh Road, Hsinchu 30010, Taiwan, ROC;Department of Biotechnology, Ming Chuan University, Taiwan, ROC and Genomic Medicine Research Core Laboratory, Chang Gung Memorial Hospital, Taiwan, ROC;Institute of Statistics, National Chiao Tung University, 1101 Ta Hsueh Road, Hsinchu 30010, Taiwan, ROC
Venue:
Journal of Biomedical Informatics
Year:
2008

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Abstract

The segmentation of cDNA microarray spots is essential in analyzing the intensities of microarray images for biological and medical investigation. In this work, nonparametric methods using kernel density estimation are applied to segment two-channel cDNA microarray images. This approach groups pixels into both a foreground and a background. The segmentation performance of this model is tested and evaluated with reference to 16 microarray data. In particular, spike genes with various contents are spotted in a microarray to examine and evaluate the accuracy of the segmentation results. Duplicated design is implemented to evaluate the accuracy of the model. The results of this study demonstrate that this method can cluster pixels and estimate statistics regarding spots with high accuracy.