Mosaicing of Fibered Fluorescence Microscopy Video

  • Authors:
  • Steve Backer;Frans Cornelissen;Jan Lemeire;Rony Nuydens;Theo Meert;Peter Schelkens;Paul Scheunders

  • Affiliations:
  • IBBT-Visionlab, Department of Physics, Antwerp University, Antwerpen, Belgium B-2610;Janssen Pharmaceutica, Beerse, Belgium B-2340;IBBT-ETRO, Vrije Universiteit Brussel, Brussel, Belgium B-1050;Janssen Pharmaceutica, Beerse, Belgium B-2340;Janssen Pharmaceutica, Beerse, Belgium B-2340;IBBT-ETRO, Vrije Universiteit Brussel, Brussel, Belgium B-1050;IBBT-Visionlab, Department of Physics, Antwerp University, Antwerpen, Belgium B-2610

  • Venue:
  • ACIVS '08 Proceedings of the 10th International Conference on Advanced Concepts for Intelligent Vision Systems
  • Year:
  • 2008

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Abstract

Fibered fluorescence microscopy is a recent developed image modality using a fiber optic probe connected to a laser scanning unit. It allows for in-vivo scanning of small animal subjects by moving the probe along the tissue surface. During the scans images are continuity captured, allowing to acquire an area larger then the field of view of the probe as a video. But there is still a need to obtain a single static image from the multiple overlapping frames. In this paper we introduce a mosaicing procedure for this kind of video sequence. An additional motivation for the mosaicing is the use of overlapping redundant information to improve the signal to noise level of the acquisition, since the individual frames tend to have both high noise levels and intensity inhomogeneities.