Quantifying the proportion of damaged sperm cells based on image analysis and neural networks

  • Authors:
  • R. Alaiz-Rodríguez;E. Alegre-Gutiérrez;V. González-Castro;L. Sánchez

  • Affiliations:
  • University of León, School of Industrial, Computing and Aeronautic Engineering, León, Spain;University of León, School of Industrial, Computing and Aeronautic Engineering, León, Spain;University of León, School of Industrial, Computing and Aeronautic Engineering, León, Spain;University of León, School of Industrial, Computing and Aeronautic Engineering, León, Spain

  • Venue:
  • SMO'08 Proceedings of the 8th conference on Simulation, modelling and optimization
  • Year:
  • 2008

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Abstract

Insemination techniques in the veterinary field demand more objective methods to control the quality of sperm samples. In particular, different factors may damage a number of sperm cells that is difficult to predict in advance. This paper addresses the problem of quantifying the proportion of damaged/intact sperm cells in a given sample based on computer vision techniques and supervised learning. Unlike common supervised classification approaches, neither the individual example classification is important nor the class distribution assumed in learning can be considered stationary. To deal with this, an estimation process based on Posterior Probability estimates (PP), and known to increase the classifier accuracy under changes in class distributions, is assessed here for quantification purposes. It is compared with two approaches based on the classifier confusion matrix (Adjusted Count and Median Sweep) and the naive approach based on classifying and counting. Experimental results with boar sperm samples and back propagation neural networks show that the PP based quantification outperforms any of the previously considered approaches in terms of the Mean Absolute Error, Kullback Leibler divergence and Mean Relative Error. Moreover, in spite of an imperfect classification, the PP approach guarantees a uniform Mean Absolute Error (around 3%) for whatever combination of training and test class distributions, what is very promising in this practical field.