Multiple Sequence Alignment System for Pyrosequencing Reads
BICoB '09 Proceedings of the 1st International Conference on Bioinformatics and Computational Biology
HCV quasispecies assembly using network flows
ISBRA'08 Proceedings of the 4th international conference on Bioinformatics research and applications
Genovo: de novo assembly for metagenomes
RECOMB'10 Proceedings of the 14th Annual international conference on Research in Computational Molecular Biology
Hi-index | 0.00 |
We present a computational method for analyzing deep sequencing data obtained from a genetically diverse sample. The set of reads obtained from a deep sequencing experiment represents a statistical sample of the underlying population. We develop a generative probabilistic model for assigning observed reads to unobserved haplotypes in the presence of sequencing errors. This clustering problem is solved in a Bayesian fashion using the Dirichlet process mixture to define a prior distribution on the unknown number of haplotypes in the mixture. We devise a Gibbs sampler for sampling from the joint posterior distribution of haplotype sequences, assignment of reads to haplotypes, and error rate of the sequencing process to obtain estimates of the local haplotype structure of the population. The method is evaluated on simulated data and on experimental deep sequencing data obtained from HIV samples.