Interpretation of gene expression microarray experiments

  • Authors:
  • Aristotelis Chatziioannou;Panagiotis Moulos

  • Affiliations:
  • Insititute of Biological Research and Biotechnology, National Hellenic Research Foundation, 48 Vassileos Constantinou ave., 11635 Athens, Greece;Insititute of Biological Research and Biotechnology, National Hellenic Research Foundation, 48 Vassileos Constantinou ave., 11635 Athens, Greece

  • Venue:
  • Proceedings of the 2007 conference on Emerging Artificial Intelligence Applications in Computer Engineering: Real Word AI Systems with Applications in eHealth, HCI, Information Retrieval and Pervasive Technologies
  • Year:
  • 2007

Quantified Score

Hi-index 0.00

Visualization

Abstract

Microarrays nowadays have an almost ubiquitous presence in modern biological research The extent and versatility of the techniques that are available for analysis and interpretation of microarray experiments can be somehow bewildering to the interested biologists. Functional genomics involves the highthroughput analysis of large datasets of information derived from various biological experiments. Microarray technology makes this possible by monitoring the emitting fluorescence reflecting the expression levels of thousands of genes simultaneously, which are bound to the oligonucleotide probes specific for each of the putative gene sequences comprising the total genome of the investigated organism, under a particular condition.. This chapter is a brief overview of the basic concepts involved in a microarray experiment; and it aspires to provide a concise overview of key issues regarding the various steps of implementation of this promising experimental methodology. In this sense, the chapter gives a feeling for what the data actually represent, and will provide information on the various computational methods that one can employ to derive meaningful results from such experiments.