Analysis of the Functional Block Involved in the Design of Radial Basis Function Networks
Neural Processing Letters
Analyzing factorial designed microarray experiments
Journal of Multivariate Analysis
A new summarization method for affymetrix probe level data
Bioinformatics
Comparative analysis of microarray normalization procedures
Bioinformatics
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Affymetrix High Oligonucleotide expression arrays, also known as Affymetrix GeneChips, are widely used for the high-throughput assessment of gene expression of thousands of genes simultaneously. Although disputed by several authors, there are non-biological variations and systematic biases that must be removed as much as possible before an absolute expression level for every gene is assessed. Several pre-processing methods are available in the literature and five common ones (RMA, GCRMA, MAS5, dChip and VSN) and two customized Loess methods are benchmarked in terms of data variability, similarity of data distributions and correlation coefficient among replicated slides in a variety of real examples. Besides, it will be checked how the variant and invariant genes can influence on preprocessing performance.