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Sugarcane cotton aphis is a common insect pest in China. Rough set and fuzzy C-means clustering were applied to identify the insect pest of sugarcane cotton aphis. Rough set was used to obtain the characteristic parameters of identify insect pest of sugarcane cotton aphis. Then, fuzzy C-means clustering was used to extract the regions of the insect pest of sugarcane cotton aphis. The experimental results showed that 17 images were segmented correctly and the accurate rate of the segmentation was 85%.