Zero-order-free image reconstruction in digital holographic microscopy

  • Authors:

  • Chandra Sekhar Seelamantula;Nicolas Pavillon;Christian Depeursinge;Michael Unser

  • Affiliations:

  • Advanced Photonics Laboratory, Ecole Polytechnique Fédérale Lausanne, Switzerland;Advanced Photonics Laboratory, Ecole Polytechnique Fédérale Lausanne, Switzerland;Advanced Photonics Laboratory, Ecole Polytechnique Fédérale Lausanne, Switzerland;Advanced Photonics Laboratory, Ecole Polytechnique Fédérale Lausanne, Switzerland

  • Venue:
  • ISBI'09 Proceedings of the Sixth IEEE international conference on Symposium on Biomedical Imaging: From Nano to Macro
  • Year:
  • 2009

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Abstract

We address the problem of zero-order-free image reconstruction in digital holographic microscopy. We show how the goal can be achieved by confining the object-wave modulation to one quadrant of the frequency domain, and by maintaining a reference-wave intensity higher than that of the object. The proposed technique is nonlinear, noniterative, and leads to exact reconstruction in the absence of noise. We also provide experimental results on holograms of yew pollen grains to validate the theoretical results.