Automatic alignment of stacks of filament data

  • Authors:
  • V. J. Dercksen;B. Weber;D. Günther;M. Oberlaender;S. Prohaska;H.-C. Hege

  • Affiliations:
  • Zuse Institute Berlin, Berlin, Germany;Zuse Institute Berlin, Berlin, Germany;Zuse Institute Berlin, Berlin, Germany;Max Planck Institute of Neurobiology, Martinsried, Germany;Zuse Institute Berlin, Berlin, Germany;Zuse Institute Berlin, Berlin, Germany

  • Venue:
  • ISBI'09 Proceedings of the Sixth IEEE international conference on Symposium on Biomedical Imaging: From Nano to Macro
  • Year:
  • 2009

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Abstract

We present a fast and robust method for the alignment of image stacks containing filamentous structures. Such stacks are usually obtained by physical sectioning a specimen, followed by an optical sectioning of each slice. For reconstruction, the filaments have to be traced and the sub-volumes aligned. Our algorithm takes traced filaments as input and matches their endpoints to find the optimal transform. We show that our method is able to quickly and accurately align sub-volumes containing neuronal processes, acquired using brightfield microscopy. Our method also makes it possible to align traced microtubuli, obtained from electron tomography data, which are extremely difficult to align manually.