3D reconstruction from electron microscope images with TXBR

Authors:
Albert F. Lawrence;Sébastien Phan;Farshid Moussavi
Affiliations:
University of California, San Diego and NCMIR, LaJolla, CA;University of California, San Diego and NCMIR, LaJolla, CA;Stanford University, Dept. of Electrical Engineering, Stanford, CA
Venue:
ISBI'09 Proceedings of the Sixth IEEE international conference on Symposium on Biomedical Imaging: From Nano to Macro
Year:
2009

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Abstract

Tomographic reconstruction from large format electron microscope requires special procedures to handle geometric distortions arising from electron optics as opposed to lightray optics. In particular, electrons travel in curvilinear paths through the sample and defocus and other aberrations can be more severe. We report on a software package, TxBR, which solves the inverse problem associated with a generalized ray transform. We also review the prospects for automated alignment, and discuss possible approaches.