Modeling of the point-spread function of laser scanning microscopes using canonical transforms

  • Authors:

  • Olle Seger;Reiner Lenz

  • Affiliations:

  • Department of Electrical Engineering, Linköping University, S-581 83 Linköping, Sweden;Department of Electrical Engineering, Linköping University, S-581 83 Linköping, Sweden

  • Venue:
  • Journal of Visual Communication and Image Representation
  • Year:
  • 1992

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Abstract

We review the theory of canonical transforms and describe how they can be used to calculate the point-spread functions of optical systems. We demonstrate their implementation in symbolic programming systems and apply the results to investigate laser scanning confocal optical microscopes. In the last part of the paper, we use the model derived to restore a 3-D image from a confocal optical microscope.