Reconstructing the optical thickness from Hoffman modulation contrast images

Authors:
Niels Holm Olsen;Jon Sporring;Mads Nielsen;Christina Hnida;Søren Ziebe
Affiliations:
3D-Lab, School of Dentistry, University of Copenhagen, Copenhagen, Denmark and IT University of Copenhagen, Copenhagen, Denmark and IH-Medical, Copenhagen, Denmark;3D-Lab, School of Dentistry, University of Copenhagen, Copenhagen, Denmark;IT University of Copenhagen, Copenhagen, Denmark;Fertility Clinic, University Hospital, Copenhagen, Denmark and IH-Medical, Copenhagen, Denmark;Fertility Clinic, University Hospital, Copenhagen, Denmark
Venue:
SCIA'03 Proceedings of the 13th Scandinavian conference on Image analysis
Year:
2003

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Abstract

Hoffman microscopy imaging systems are part of numerous fertility clinics world-wide. We discuss the physics of the Hoffman imaging system from optical thickness to image intensity, implement a simple, yet fast, reconstruction algorithm using Fast Fourier Transformation and discuss the usability of the method on a number of cells from a human embryo. Novelty is identifying the non-linearity of a typical Hoffman imaging system, and the application of Fourier Transformation to reconstruct the optical thickness.