Constructing the histogram representation for automatic gridding of cDNA microarray images

Authors:
Hong-Qiang Wang;Hau-San Wong;Hailong Zhu
Affiliations:
Department of RIIPT, The Hong Kong Ploytechnic University, Hong Kong, China;Department of Computer Science, City University of Hong Kong, Hong Kong, China;Department of RIIPT, The Hong Kong Ploytechnic University, Hong Kong, China
Venue:
ICMB'08 Proceedings of the 1st international conference on Medical biometrics
Year:
2008

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Abstract

This paper proposes an novel approach for automatic gridding of cDNA microarray images based on histogram representation. The approach constructs histogram representation to characterize microarray images and use it, instead of raw signal intensities used in previous gridding methods, to identify spots. The histogram representation can efficiently reduce noise and the influence from low raw signal intensities and local contaminations. The proposed approach is successfully tested on different types of microarray images and is compared with several previous algorithms.