De novo sequencing of nonribosomal peptides

Authors:
Nuno Bandeira;Julio Ng;Dario Meluzzi;Roger G. Linington;Pieter Dorrestein;Pavel A. Pevzner
Affiliations:
University of California, San Diego;University of California, San Diego;University of California, San Diego;University of California, Santa Cruz;University of California, San Diego;University of California, San Diego
Venue:
RECOMB'08 Proceedings of the 12th annual international conference on Research in computational molecular biology
Year:
2008

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Abstract

While nonribosomal peptides (NRPs) are of tremendous pharmacological importance, there is currently no technology capable of high-throughput sequencing of NRPs. Difficulties in sequencing NRPs slow down the progress in elucidating the non-ribosomal genetic code and negatively affect various screening programs aimed at the discovery of natural compounds of medical importance. We propose to employ multi-stage mass-spectrometry (MSn) for the data acquisition, followed by alignment-based heuristic algorithms for data analysis. Since mass spectrometry based analysis of NRPs is fast and inexpensive, this approach opens the possibility of high-throughput sequencing of many unknown NRPs accumulated in large screening programs.