A vesselness-guided variational segmentation of cellular networks from 3D micro-CT

  • Authors:
  • Alexandra Pacureanu;Chantal Revol-Muller;Jean-Loïc Rose;Maria Sanchez Ruiz;Françoise Peyrin

  • Affiliations:
  • CREATIS-LRMN, INSA-Lyon, Insenn, CNRS, UMR, Université de Lyon, Lyon, France and ESRF, Grenoble Cedex;CREATIS-LRMN, INSA-Lyon, Insenn, CNRS, UMR, Université de Lyon, Lyon, France;CREATIS-LRMN, INSA-Lyon, Insenn, CNRS, UMR, Université de Lyon, Lyon, France;CREATIS-LRMN, INSA-Lyon, Insenn, CNRS, UMR, Université de Lyon, Lyon, France;CREATIS-LRMN, INSA-Lyon, Insenn, CNRS, UMR, Université de Lyon, Lyon, France and ESRF, Grenoble Cedex

  • Venue:
  • ISBI'10 Proceedings of the 2010 IEEE international conference on Biomedical imaging: from nano to Macro
  • Year:
  • 2010

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Abstract

Advances in imaging techniques lead to nondestructive 3D visualization of biological tissue at a sub-cellular scale. As a consequence, new demands emerge to segment complex structures. For instance, synchrotron radiation micro-CT, makes it possible to image the lacunar-canalicular porosity in bone tissue. This structure contains a dense network of slender channels interconnecting the cells. Their size (-300- 600 nanometers in diameter) is at the limit of the acquisition system resolution (280nm) making their detection difficult. In this work is proposed a variational region growing segmentation method adapted for cellular networks. To control the evolution of the segmentation through tubular structures a vesselness map is introduced in the expression of the functional to minimize. The method is tested on synthetic images and applied to experimental data.