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This paper investigates the work of creating infrastructure, using as a case study the development of cyberinfrastructure for metagenomics research. Specifically, the analysis focuses on the role of embeddedness in infrastructure development. We expand on the notion of human infrastructure to develop the concepts of synergizing, leveraging, and aligning, which denote the active processes of creating and managing relationships among people, organizations, and technologies in the creation of cyberinfrastructure. This conceptual lens highlights how embeddedness is not only an important result of infrastructure development, but is also a precursor that can act as both a constraint and a resource for development activities.