Analysis of length and orientation of microtubules in wide-field fluorescence microscopy

Authors:
Gerlind Herberich;Anca Ivanescu;Ivonne Gamper;Antonio Sechi;Til Aach
Affiliations:
Institute of Imaging and Computer Vision, RWTH Aachen University, Germany;Institute of Imaging and Computer Vision, RWTH Aachen University, Germany;Institute of Biomedical Engineering Dept. of Cell Biology, RWTH Aachen University, Germany;Institute of Biomedical Engineering Dept. of Cell Biology, RWTH Aachen University, Germany;Institute of Imaging and Computer Vision, RWTH Aachen University, Germany
Venue:
Proceedings of the 32nd DAGM conference on Pattern recognition
Year:
2010

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Abstract

In this paper we present a novel approach for the analysis of microtubules in wide-field fluorescence microscopy. Microtubules are flexible elongated structures and part of the cytoskeleton, a cytoplasmic scaffolding responsible for cell stability and motility. The method allows for precise measurements of microtubule length and orientation under different conditions despite a high variability of image data and in the presence of artefacts. Application of the proposed method to demonstrate the effect of the protein GAR22 on the rate of polymerisation of microtubules illustrates the potential of our approach.