A Multiphase Dynamic Labeling Model for Variational Recognition-driven Image Segmentation
International Journal of Computer Vision
Tracking the Swimming Motions of C. elegans Worms with Applications in Aging Studies
MICCAI '08 Proceedings of the 11th international conference on Medical Image Computing and Computer-Assisted Intervention - Part I
Resolving clustered worms via probabilistic shape models
ISBI'10 Proceedings of the 2010 IEEE international conference on Biomedical imaging: from nano to Macro
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We present a novel approach for extracting cluttered objects based on their morphological properties. Specifically, we address the problem of untangling Caenorhabditis elegans clusters in high-throughput screening experiments. We represent the skeleton of each worm cluster by a sparse directed graph whose vertices and edges correspond to worm segments and their adjacencies, respectively. We then search for paths in the graph that are most likely to represent worms while minimizing overlap. The worm likelihood measure is defined on a low-dimensional feature space that captures different worm poses, obtained from a training set of isolated worms. We test the algorithm on 236 microscopy images, each containing 15 C. elegans worms, and demonstrate successful cluster untangling and high worm detection accuracy.