Proceedings of the International Conference on Computer-Aided Design
Sample preparation for many-reactant bioassay on DMFBs using common dilution operation sharing
Proceedings of the International Conference on Computer-Aided Design
Optimization of polymerase chain reaction on a cyberphysical digital microfluidic biochip
Proceedings of the International Conference on Computer-Aided Design
Hi-index | 0.01 |
A biochemical analysis is based on several laboratory protocols that require repeated mixing of samples with reagents. Sample preparation and analyte identification steps in such bioassays often involve mixing for solution preparation, i.e., various fluids are to be mixed in a certain volumetric ratio in their resulting mixture. We present an efficient approach for automated mixing of three or more fluids on a droplet based digital micro fluidic biochip and design a layout for implementing this algorithm. The proposed method reduces the droplet transportation time from boundary reservoirs to on chip mixers as well as cross-contamination among overlapping droplet routing paths. Simulation of several example solutions reveals encouraging results.