A calculus for the random generation of labelled combinatorial structures
Theoretical Computer Science
Energy landscape of k-point mutants of an RNA molecule
Bioinformatics
Controlled non-uniform random generation of decomposable structures
Theoretical Computer Science
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The analysis of the impact of mutations on folding properties of RNAs is essential to decipher principles driving molecular evolution and to design new molecules. We recently introduced an algorithm called RNAmutants which samples RNA sequence-structure maps in polynomial time and space. However, since the mutation probabilities depend of the free energy of the structures, RNAmutants is bias toward G+C-rich regions of the mutational landscape. In this paper we introduce an unbiased adaptive sampling algorithm that enables RNAmutants to sample regions of the mutational landscape poorly covered by previous techniques. We applied the method to sample mutations in complete RNA sequencestructures maps of sizes up to 40 nucleotides. Our results indicate that the G+C-content has a strong influence on the evolutionary accessible structural ensembles. In particular, we show that low G+C-contents favor the apparition of internal loops, while high G+C-contents reduce the size of the evolutionary accessible mutational landscapes.