Marching cubes: A high resolution 3D surface construction algorithm
SIGGRAPH '87 Proceedings of the 14th annual conference on Computer graphics and interactive techniques
Performance Evaluation of Some Clustering Algorithms and Validity Indices
IEEE Transactions on Pattern Analysis and Machine Intelligence
An Efficient Algorithm for Optimal Multilevel Thresholding of Irregularly Sampled Histograms
SSPR & SPR '08 Proceedings of the 2008 Joint IAPR International Workshop on Structural, Syntactic, and Statistical Pattern Recognition
A multi-level thresholding-based method to learn fuzzy membership functions from data warehouse
CIARP'11 Proceedings of the 16th Iberoamerican Congress conference on Progress in Pattern Recognition, Image Analysis, Computer Vision, and Applications
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The appreciation of biofilm structures in digital images can be subjective to the observer, and hence it is necessary to analyse the underlying images in useful parameters by means of quantification that is, ideally, free of errors. This paper proposes a combination of techniques for segmentation of biofilm images through an optimal multi-level thresholding algorithm and a set of clustering validity indices, including the determination of the best number of thresholds. The results, which are validated through Rand Index and a quantification process performed in a laboratory, are similar to the quantification and segmentation done by an expert.