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This paper emphasize the role of ontologies in the e-Health domain, namely in Occupational Health. It describes (by means of a case study) the representation and integration of the semantic and modelling layers of a system based on ontologies for the prevention of occupational risks, as an application of ontologies in the occupational health domain. The case study enumerates basic concepts and relationships in the proposed reference ontology and model, as well as the basic reasoning for them. The personalized training on the health, safety, security and environmental issues is related to the work activities. The training is based on the automatic and semantics-based discovery of the prevention documents, actions, methods, etc that fit the user's request.