Automatic extraction of DNA profiles in polyacrilamide gel electrophoresis images

  • Authors:
  • Francisco Silva-Mata;Isneri Talavera-Bustamante;Ricardo González-Gazapo;Noslén Hernández-González;Juan R. Palau-Infante;Marta Santiesteban-Vidal

  • Affiliations:
  • Advanced Technologies Applications Center, MINBAS, Cuba;Advanced Technologies Applications Center, MINBAS, Cuba;Advanced Technologies Applications Center, MINBAS, Cuba;Advanced Technologies Applications Center, MINBAS, Cuba;Advanced Technologies Applications Center, MINBAS, Cuba;Central Criminologist Laboratory, Cuba

  • Venue:
  • CIARP'05 Proceedings of the 10th Iberoamerican Congress conference on Progress in Pattern Recognition, Image Analysis and Applications
  • Year:
  • 2005

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Abstract

In this paper is presented a method for the automatic DNA spots classification and extraction of profiles associated in DNA polyacrilamide gel electrophoresis based on image processing. A software which implements this method was developed, composed by four modules: Digital image acquisition, image preprocessing, feature extraction and classification, and DNA profile extraction. The use of different types of algorithms as: C4.5 Decision Trees, Support Vector Machines and Leader Algorithm are needed to resolve all the tasks. The experimental results show that this method has a very nice computational behavior and effectiveness, and provide a very useful tool to decrease the time and increase the quality of the specialist responses.