Development of a new index to evaluate zooplanktons’ gonads: an approach based on a suitable combination of deformable models

Authors:
M. Ramiro Pastorinho;Miguel A. Guevara;Augusto Silva;Luis Coelho;Fernando Morgado
Affiliations:
Biology Department, University of Aveiro, Aveiro, Portugal;Computer Sciences Faculty, University of Ciego de Avila, Ciego de Avila, Cuba;IEETA, University of Aveiro, Aveiro, Portugal;IEETA, University of Aveiro, Aveiro, Portugal;Biology Department, University of Aveiro, Aveiro, Portugal
Venue:
CIARP'05 Proceedings of the 10th Iberoamerican Congress conference on Progress in Pattern Recognition, Image Analysis and Applications
Year:
2005

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Abstract

Acartia tonsa was used as model to establish an index for oocyte maturity determination in zooplankters based in citometry and histochemical evaluation of gonadic masses. Biometry was performed using an ocular micrometer and nucleus/cytoplasm ratios were obtained characterizing each of the three identified stages: Immature, Vitellogenic and Mature. This paper presents a novel approach since it joins (and, indeed, reinforces) the index framework with the evaluation of the same biological samples by a suitable combination of deformable models. Nucleus contour is identified through Active Shape Models techniques, and cytoplasm contour’s detected through parametric Snakes, with prior image preprocessing based on statistical and mathematical morphology techniques. Morphometric parameters such as nucleus and cytoplasm area and ratio between them are then easily computed. As a result the dataset validated the applied methodology with a realistic background and a new, more accurate and ecologically realistic index for oocyte staging emerged.