Principles of multivariate analysis: a user's perspective
Principles of multivariate analysis: a user's perspective
Fundamentals of digital image processing
Fundamentals of digital image processing
Handbook of pattern recognition & computer vision
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IEEE Transactions on Pattern Analysis and Machine Intelligence
Robust computer vision: an interdisciplinary challenge
Computer Vision and Image Understanding - Special issue on robusst statistical techniques in image understanding
Mean Shift: A Robust Approach Toward Feature Space Analysis
IEEE Transactions on Pattern Analysis and Machine Intelligence
Fractal analysis of tumor in brain MR images
Machine Vision and Applications
MaZda-A software package for image texture analysis
Computer Methods and Programs in Biomedicine
Fractal features for localization of temporal lobe epileptic foci using SPECT imaging
Computers in Biology and Medicine
Texture classification using kernel-based techniques
IWANN'13 Proceedings of the 12th international conference on Artificial Neural Networks: advances in computational intelligence - Volume Part I
Teeth segmentation of dental periapical radiographs based on local singularity analysis
Computer Methods and Programs in Biomedicine
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Texture analysis provides a means to quantify complex changes in microscope images. We previously showed that cytoplasmic poly-adenylated mRNAs form mRNA granules in post-ischemic neurons and that these granules correlated with protein synthesis inhibition and hence cell death. Here we utilized the texture analysis software MaZda to quantify mRNA granules in photomicrographs of the pyramidal cell layer of rat hippocampal region CA3 around 1h of reperfusion after 10min of normothermic global cerebral ischemia. At 1h reperfusion, we observed variations in the texture of mRNA granules amongst samples that were readily quantified by texture analysis. Individual sample variation was consistent with the interpretation that animal-to-animal variations in mRNA granules reflected the time-course of mRNA granule formation. We also used texture analysis to quantify the effect of cycloheximide, given either before or after brain ischemia, on mRNA granules. If administered before ischemia, cycloheximide inhibited mRNA granule formation, but if administered after ischemia did not prevent mRNA granulation, indicating mRNA granule formation is dependent on dissociation of polysomes. We conclude that texture analysis is an effective means for quantifying the complex morphological changes induced in neurons by brain ischemia and reperfusion.