Rapid automated three-dimensional tracing of neurons from confocal image stacks
IEEE Transactions on Information Technology in Biomedicine
A Pyramidal Neural Network For Visual Pattern Recognition
IEEE Transactions on Neural Networks
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The developed model consists of a multilayer neural network with receptive fields used to estimate the local direction of the neuron on a fragment of microscopy image. It can be used in a wide range of classical neuron reconstruction methods (manual, semi-automatic, local automatic or global automatic), some of which are also outlined in this paper. The model is trained on an automatically generated training set extracted from a provided example image stack and corresponding reconstruction file. During the experiments the model was tested in simple statistical tests and in real applications, and achieved good results. The main advantage of the proposed approach is its simplicity for the end-user, one who might have little or no mathematical/computer science background, as it does not require any manual configuration of constants.