Numerical recipes in C (2nd ed.): the art of scientific computing
Numerical recipes in C (2nd ed.): the art of scientific computing
Morphological Image Analysis: Principles and Applications
Morphological Image Analysis: Principles and Applications
On the Use of Distance Maps in the Analysis of 1D DNA Gel Images
ICDIP '09 Proceedings of the International Conference on Digital Image Processing
An alternative method for electrophoretic gel image analysis in the GelMaster software
Computer Methods and Programs in Biomedicine
Automatic Method to Compare the Lanes in Gel Electrophoresis Images
IEEE Transactions on Information Technology in Biomedicine
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This paper proposes a method for automating the detection of lanes in Thin-Layer Chromatography images. Our approach includes a preprocessing step to detect the image region of interest, followed by background estimation and removal. This image is then projected onto the horizontal direction to integrate the information into a one-dimensional profile. A smoothing filter is applied to this profile and the outcome is the input of the lane detection process, which is performed in three phases. The first one aims at obtaining an initial set of candidate lanes that are further validated or removed in the second phase. The last phase is a refinement step that allows the inclusion of lanes that are not clearly distinguishable in the profile and that were not included in the initial set. The method was evaluated in 66 chromatography images and achieved values of recall, precision and Fβ-measure of 97.0%, 99.4% and 98.2%, respectively.