Distinctive Image Features from Scale-Invariant Keypoints
International Journal of Computer Vision
Morphological multiscale decomposition of connected regions with emphasis on cell clusters
Computer Vision and Image Understanding
Automated Arabidopsis plant root cell segmentation based on SVM classification and region merging
Computers in Biology and Medicine
Automatic Segmentation of High-Throughput RNAi Fluorescent Cellular Images
IEEE Transactions on Information Technology in Biomedicine
Gradient convergence filters and a phase congruency approach for in vivo cell nuclei detection
Machine Vision and Applications - Special Issue: Microscopy Image Analysis for Biomedical Applications
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Evaluation of parasite infection indexes on in vitro cell cultures is a practice commonly employed by biomedical researchers to address biological questions or to test the efficacy of novel anti-parasitic compounds. In the particular case of Leishmania infantum, a unicellular parasite that parasitizes macrophages, infection indexes are usually determined either by visual inspection of cells directly under the microscope or by counting digital images using appropriate software. In either case assessment of infection indexes is time consuming, thus motivating the creation of automatic image analysis approaches that allow large scale studies of Leishmania-infected macrophage cultures. We propose a fully automated method for automatic evaluation of parasite infection indexes through the segmentation of individual macrophages nucleus and cytoplasm, as well as the segmentation and co-localization of the parasites in the image. To perform such analysis with robustness and increased performance we propose the use of local image filters tuned to the specific size of the objects to detect, in conjunction with image segmentation approaches. The objects size estimation is then improved through a learning feedback loop. Cytoplasm is detected by seeded watershed segmentation. Our approach obtains, for 86 images from 4 experiments, an average parasite infection index evaluation error of 2.3%.