3D optical microscopy method based on synthetic aperture integral imaging

  • Authors:
  • Joon-Jae Lee;Donghak Shin;Byung-Gook Lee;Hoon Yoo

  • Affiliations:
  • Dept. of Game Mobile Contents, Keimyung University, Daegu, Korea 705-701;Institute of Ambient Intelligence, Dongseo University, Busan, Korea 617-716;Institute of Ambient Intelligence, Dongseo University, Busan, Korea 617-716;Div. of Digital Media Technology, Sangmyung University, Seoul, Korea 110-743

  • Venue:
  • 3D Research
  • Year:
  • 2012

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Abstract

In this paper, we propose a 3D optical microscopy system based on synthetic aperture integral imaging (SAII) and apply it to a surface extraction application. In the proposed system, the micro-object is optically magnified in optical microscope system and the elemental images of magnified micro-object are recorded using the SAII sensing method. The recorded elemental images are used to reconstruct a set of 3D slice images using the computational reconstruction algorithm based on ray back-projection. We obtain the surface extraction of micro-object based on the estimation of depth by using the block matching algorithm between the elemental image and a set of the reconstructed 3D slice images. The longitudinal and lateral resolutions are analyzed for the proposed system. To demonstrate our system, we carry out the preliminary experiments of a micro-object and the results are presented.