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As well-known, the science of acupuncture and moxibustion is an important component of Traditional Chinese Medicine with a long history. Although there are a number of different acupuncture manipulations, the method for distinguishing them is rarely investigated. With the idea of the interspike interval (ISI), we study the electrical signal time series at the spinal dorsal horn produced by three different acupuncture manipulations in Zusanli point and present an effective way to distinguish them. Comparing with the traditional analysis methods, like phase space reconstruction and largest Lyapunov exponents, this new method is more efficiently and effective.