Processing random amplified polymorphysm DNA images using the radon transform and mathematical morphology

Authors:
Luis Rueda;Omar Uyarte;Sofia Valenzuela;Jaime Rodriguez
Affiliations:
Department of Computer Science and Laboratory for Forest Genomics, Center for Biotechnology, University of Concepción, Concepción, Chile;Laboratory for Forest Genomics, Center for Biotechnology, University of Concepción, Chile;Department of Forest Science and Laboratory for Forest Genomics, Center for Biotechnology, University of Concepción, Chile;Department of Forest Science and Laboratory for Forest Genomics, Center for Biotechnology, University of Concepción, Chile
Venue:
ICIAR'07 Proceedings of the 4th international conference on Image Analysis and Recognition
Year:
2007

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Abstract

Random Amplified Polymorphism DNA (RAPD) analysis is a wellknown method for studying genetic relationships between individuals. In this context, processing the underlying RAPD images is a quite difficult problem, affected by various factors. A method for processing RAPD images is proposed, which aims to detect bands by pre-processing the images by performing a template correction step and a band detection mechanism. The results on RAPD images, which aim to verify genetic identity among tree individuals, show that the proposed method detects either negative or positive amplification (bands) with a sensitivity of over 94%.