Learning to detect cells using non-overlapping extremal regions

  • Authors:
  • Carlos Arteta;Victor Lempitsky;J. Alison Noble;Andrew Zisserman

  • Affiliations:
  • Department of Engineering Science, University of Oxford, U.K.;Yandex, Moscow, Russia;Department of Engineering Science, University of Oxford, U.K.;Department of Engineering Science, University of Oxford, U.K.

  • Venue:
  • MICCAI'12 Proceedings of the 15th international conference on Medical Image Computing and Computer-Assisted Intervention - Volume Part I
  • Year:
  • 2012

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Abstract

Cell detection in microscopy images is an important step in the automation of cell based-experiments. We propose a machine learning-based cell detection method applicable to different modalities. The method consists of three steps: first, a set of candidate cell-like regions is identified. Then, each candidate region is evaluated using a statistical model of the cell appearance. Finally, dynamic programming picks a set of non-overlapping regions that match the model. The cell model requires few images with simple dot annotation for training and can be learned within a structured SVM framework. In the reported experiments, state-of-the-art cell detection accuracy is achieved for H&E-stained histology, fluorescence, and phase-contrast images.