Application of image processing algorithms in proteomics: automatic analysis of 2-d gel electrophoresis images from western blot assay

Authors:
Katarzyna Jonak;Karol Jędrasiak;Andrzej Polański;Krzysztof Puszyński
Affiliations:
Institute of Automatic Control, Silesian University of Technology, Gliwice, Poland;Institute of Automatic Control, Silesian University of Technology, Gliwice, Poland,Polish-Japanese Institute of Information Technollogy, Bytom, Poland;Institute of Automatic Control, Silesian University of Technology, Gliwice, Poland,Polish-Japanese Institute of Information Technollogy, Bytom, Poland;Institute of Automatic Control, Silesian University of Technology, Gliwice, Poland
Venue:
ICCVG'12 Proceedings of the 2012 international conference on Computer Vision and Graphics
Year:
2012

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Abstract

Studying changes in the cell after treatment by a potentially genotoxic agent is a very important approach in biological experimental techniques. A researcher in molecular biology can study the structure of cellular pathways related to responses to external agents stress, damage or ionizing radiation by measuring the amount of cellular species, such as certain proteins, RNA or DNA. For this aim the electrophoresis-based tests are widely used. In this paper we will focus on the Western blot assay for estimating the quantity of the proteins. Often such information is obtained by visual analysis of a gel, which is prejudicial and time-consuming. We developed a new, rapid and exact image processing method for automatic detection of the spots in 2-D gels and calculation the quantity of the protein produced by the cell. The proposed method can significantly reduce the time required for analysis. We have obtained very promising results with accuracy more than 96% allowing for automated analyzes of 2-D gel electrophoresis images.