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Reader-to-reader interference significantly affects the performance of RFID applications. The design of an RFID system should carefully consider this phenomenon. Simulation can dramatically speed up the design and testing phase, by deferring the implementation of a prototype to the last phase of the development. Unfortunately, no specific simulators of interference in RFID networks are currently available. Previous works exploited either general purpose network simulators, which often do not provide the required features for simulating an RFID network, or self deployed tools, which are not publicly available and therefore do not allow the validation and reproducibility of the results. This paper identifies the requirements that a simulator of reader-to-reader interference should satisfy and presents the R2RIS simulator, which has been specifically designed to evaluate the performance of reader-to-reader anti-collision protocols.