Cell Migration Analysis After In Vitro WoundingInjury with a Multi-Agent Approach

  • Authors:
  • Alain Boucher;Anne Doisy;Xavier Ronot;Catherine Garbay

  • Affiliations:
  • Laboratoire TIMC/IMAG, Grenoble, France;DyOGen (UPRES n° EA 2021), INSERM U309, Grenoble, France and Laboratoire de Neurobiologie du Développement, E.P.H.E., Montpellier, France (Authors can be contacted at: Institut Albert ...;DyOGen (UPRES n° EA 2021), INSERM U309, Grenoble, France and Laboratoire de Neurobiologie du Développement, E.P.H.E., Montpellier, France (Authors can be contacted at: Institut Albert ...;Laboratoire TIMC/IMAG, Grenoble, France

  • Venue:
  • Artificial Intelligence Review
  • Year:
  • 1998

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Abstract

This paper presents a multiagent system for studying in vitro cellmotion. A typical application on the wound closure process is presented toillustrate the possibilities of the system, where different image sequenceswill be treated. The motion issue involves three aspects: imagesegmentation, object tracking and motion analysis. The current systemversion focuses mainly on the image segmentation aspect. A general agentmodel has been designed, which will be further expanded to include trackingand motion analysis behaviors as well. The agents integrate three basicbehaviors: perception, interaction and reproduction. The perceptionevaluates pixels upon static and motion-based criteria. The interactionbehavior allows two agents to merge or to negotiate parts of regions. Thenegotiation can be seen as a segmentation refinement process done by the agents. Finally, the reproduction behavior defines an exploration strategyof the images. Agents can start other agents around them, or they canduplicate themselves in the next frame. The frames are processed inpipeline, where previous information is used to treat the current frame.One unique agent model exist. Agents are specialized on execution timeaccording to their goals. The results, coming from an existing prototype,show different types of cell behavior during cell migration, based on cellnuclei analysis.