Discrete-time signal processing
Discrete-time signal processing
RECOMB '00 Proceedings of the fourth annual international conference on Computational molecular biology
Protein structure determination using protein threading and sparse NMR data (extended abstract)
RECOMB '00 Proceedings of the fourth annual international conference on Computational molecular biology
Blind inverse gamma correction
IEEE Transactions on Image Processing
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High-throughput, data-directed computational protocols for Structural Genomics (or Proteomics) are required in order to evaluate the protein products of genes for structure and function at rates comparable to current gene-sequencing technology. To develop such methods, new algorithms are required that can quickly extract significantly more structural information from sparse experimental data. This paper presents a new class of signal processing algorithms for nuclear magnetic resonance (NMR) structural biology, based on time-frequency analysis of chemical shift dynamics.A novel approach to multidimensional NMR analysis is proposed in which the data are interpreted in the time-frequency domain, as opposed to the traditional frequency domain. Time-frequency analysis (TFA) exposes behavior orthogonal to the magnetic coherence transfer pathways, thus affording new avenues of NMR discovery. An implementation yielding new biophysical results is discussed. In particular, we demonstrate the heretofore unknown presence of through-space inter-atomic distance information within 15N-edited heteronuclear single-quantum coherence(15N HSQC) data. A biophysical model explains these results, and is supported by further experiments on simulated spectra.