Refining Restriction Enzyme Genome Maps

  • Authors:
  • Peter Z. Revesz

  • Affiliations:
  • Department of Computer Science and Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588-0115, USA

  • Venue:
  • Constraints
  • Year:
  • 1998

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Abstract

A genome map is an ordering of a set of clones accordingto their believed position on a DNA string. Simple heuristicsfor genome map assembly based on single restriction enzyme withcomplete digestion data can lead to inaccuracies and ambiguities.This paper presents a method that adds additional constraintchecking to the assembly process. An automaton is presented thatfor any genome map produces a refined genome map where both theclones and the restriction fragments in each clone are orderedsatisfying natural constraints called step constraints. Any genomemap that cannot be refined is highly likely to be inaccurateand can be eliminated as a possibility.