A hybrid approach to the 3D high precision position measurement and particle tracking in human cell nuclei

  • Authors:

  • A. Cavallo;I. Solovei;L. Schermeleh;R. Heintzmann;T. Cremer;C. Cremer

  • Affiliations:

  • Interdisziplinäres Zentrum für Wissenschaftliches Rechnen, Kirchhoff-Institut für Physik, Germany;Institute of Anthropology and Human Genetics university of München, Germany;Institute of Anthropology and Human Genetics university of München, Germany;Kirchhoff-Institut für Physik, Germany;Institute of Anthropology and Human Genetics university of München, Germany;Interdisziplinäres Zentrum für Wissenschaftliches Rechnen, Kirchhoff-Institut für Physik, Germany

  • Venue:
  • Real-Time Imaging - Special issue: Imaging in bioinformatics part II
  • Year:
  • 2002

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Abstract

In this report, we present a detection and tracking system for fluorescent particles inside a cell nucleus. We present the application of this system to a real biological problem where the same human cell nucleus is scanned in three different states: living, fixed and after a treatment used for fluorescence in situ hybridization (FISH). The change in distances between internal subchromosomal structures was analyzed.