Estimating Seed Sensitivity on Homogeneous Alignments

  • Authors:
  • Affiliations:
  • Venue:
  • BIBE '04 Proceedings of the 4th IEEE Symposium on Bioinformatics and Bioengineering
  • Year:
  • 2004

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Abstract

We address the problem of estimating the sensitivity ofseed-based similarity search algorithms. In contrast to approachesbased on Markov models [Faster and more sensitive homology search, Designing seeds for similarity search in genomic DNA, Optimal spaced seeds for Hidden Markov Models, with application to homologous coding regions, Vector seeds: an extension to spaced seeds allows substantial improvements in sensitivity and specificity, Sensitivity analysis and efficient method for identifying optimal spaced seeds], we studythe estimation based on homogeneous alignments. We describean algorithm for counting and random generation ofthose alignments and an algorithm for exact computation ofthe sensitivity for a broad class of seed strategies. We provideexperimental results demonstrating a bias introducedby ignoring the homogeneousness condition.