IEEE Transactions on Pattern Analysis and Machine Intelligence
Kernel Designs for Efficient Multiresolution Edge Detection and Orientation Estimation
IEEE Transactions on Pattern Analysis and Machine Intelligence
Computer graphics (2nd ed. in C): principles and practice
Computer graphics (2nd ed. in C): principles and practice
Finding circles by an array of accumulators
Communications of the ACM
Use of the Hough transformation to detect lines and curves in pictures
Communications of the ACM
Computer and Robot Vision
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This paper presents a method for the detection andmeasurement of inhibition halos of antibacterialactivity. It is a difficult problem because there is awide range of non-homogeneous culture mediums,with a wide range of contrasts between backgroundand halos and some halos have diffuse and irregularor discontinuous contours. In addition, the size,position and number of halos are variable andunforeseeable. The technique exploits the circularityof both the target bacteria and the surrounding halosand uses the Hough Transform to detect multipleconcentric circles. In order to reduce time andmemory space the circle detection with the HT isseparated in two stages. Several HT center detectionmethods were tested. Prior to the halos detection theimages are preprocessed to remove noise and locatethe region of interest. Experimental results show theeffectiveness of the proposed technique on differentculture mediums.