Probabilistic nucleotide assembling method for sequencing by hybridization

  • Authors:
  • Takaho A. Endo

  • Affiliations:
  • Division of Molecular Life Sciences, Department of Genetic Information, School of Medicine, Tokai University, Bouseidai, Isehara, Kanagawa, 259-1193, Japan

  • Venue:
  • Bioinformatics
  • Year:
  • 2004

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Abstract

Motivation: Developing a new method of assembling small sequences based on sequencing by hybridization with many positive and negative faults. First, an interpretation of a generic traveling salesman problem is provided (i.e. finding the shortest route for visiting many cities), using genetic algorithms. Second, positive errors are excluded before assembly by a sanitization process. Results: The present method outperforms those described in previous studies, in terms of both time and accuracy. Availability: http://kamit.med.u-tokai.ac.jp/~takaho/sbh/index.html