G-PRIMER: greedy algorithm for selecting minimal primer set

Authors:
Jiren Wang;Kuo-Bin Li;Wing-Kin Sung
Affiliations:
BioInformatics Institute, 30 Biopolis Street, #07-01 Matrix, Singapore 138671;BioInformatics Institute, 30 Biopolis Street, #07-01 Matrix, Singapore 138671;Department of Computer Science, National University of Singapore, 3 Science Drive 2, Singapore 117543
Venue:
Bioinformatics
Year:
2004

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Abstract

Summary: G-PRIMER, a web-based primer design program, has been developed to compute a minimal primer set specifically annealed to all the open reading frames in a given microbial genome. This program has been successfully used in the microarray experiment for analyzing the expression of genes in the Xanthomonas campestris genome. Availability: It is available at http://mammoth.bii.a-star.edu.sg/gprimer/. Its source code is available upon request.