Motif discovery in tissue-specific regulatory sequences using directed information
EURASIP Journal on Bioinformatics and Systems Biology
Waiting time distribution of generalized later patterns
Computational Statistics & Data Analysis
GPU-MEME: Using Graphics Hardware to Accelerate Motif Finding in DNA Sequences
PRIB '08 Proceedings of the Third IAPR International Conference on Pattern Recognition in Bioinformatics
Pattern Recognition Letters
Computational molecular biology of genome expression and regulation
PReMI'05 Proceedings of the First international conference on Pattern Recognition and Machine Intelligence
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Motivation: Tissue-specific transcription factor binding sites give insight into tissue-specific transcription regulation. Results: We describe a word-counting-based tool for de novo tissue-specific transcription factor binding site discovery using expression information in addition to sequence information. We incorporate tissue-specific gene expression through gene classification to positive expression and repressed expression. We present a direct statistical approach to find overrepresented transcription factor binding sites in a foreground promoter sequence set against a background promoter sequence set. Our approach naturally extends to synergistic transcription factor binding site search. We find putative transcription factor binding sites that are overrepresented in the proximal promoters of liver-specific genes relative to proximal promoters of liver-independent genes. Our results indicate that binding sites for hepatocyte nuclear factors (especially HNF-1 and HNF-4) and CCAAT/enhancer-binding protein (C/EBPβ) are the most overrepresented in proximal promoters of liver-specific genes. Our results suggest that HNF-4 has strong synergistic relationships with HNF-1, HNF-4 and HNF-3β and with C/EBPβ. Availability: Programs are available for use over the Web at http://rulai.cshl.edu/tools/dwe Contact:ps@cs.pdx.edu; mzhang@cshl.edu Supplementary information: Data and omitted results are available at http://rulai.cshl.edu/tools/dwe/supp