MultiPLX: automatic grouping and evaluation of PCR primers

  • Authors:

  • Lauris Kaplinski;Reidar Andreson;Tarmo Puurand;Maido Remm

  • Affiliations:

  • Department of Bioinformatics, University of Tartu Tartu, Estonia;Department of Bioinformatics, University of Tartu Tartu, Estonia;Department of Bioinformatics, University of Tartu Tartu, Estonia;Department of Bioinformatics, University of Tartu Tartu, Estonia

  • Venue:
  • Bioinformatics
  • Year:
  • 2005

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Abstract

Summary: MultiPLX is a new program for automatic grouping of PCR primers. It can use many different parameters to estimate the compatibility of primers, such as primer--primer interactions, primer--product interactions, difference in melting temperatures, difference in product length and the risk of generating alternative products from the template. A unique feature of the MultiPLX is the ability to perform automatic grouping of large number (thousands) of primer pairs. Availability: Binaries for Windows, Linux and Solaris are available from http://bioinfo.ebc.ee/download/. A graphical version with limited capabilities can be used through a web interface at http://bioinfo.ebc.ee/multiplx/. The source code of the program is available on request for academic users. Contact: maido.remm@ut.ee