Using transformation knowledge for the classification of Raman spectra of biological samples

  • Authors:
  • Klaus.-D. Peschke;Bernard Haasdonk;Olaf Ronneberger;Hans Burkhardt;Petra Rösch;Michaela Harz;Jürgen Popp

  • Affiliations:
  • Lehrstuhl für Mustererkennung und Bildverarbeitung, Institut für Informatik, Albert-Ludwigs-Universität Freiburg, Georges-Köhler-Allee, Freiburg, Germany;Lehrstuhl für Mustererkennung und Bildverarbeitung, Institut für Informatik, Albert-Ludwigs-Universität Freiburg, Georges-Köhler-Allee, Freiburg, Germany;Lehrstuhl für Mustererkennung und Bildverarbeitung, Institut für Informatik, Albert-Ludwigs-Universität Freiburg, Georges-Köhler-Allee, Freiburg, Germany;Lehrstuhl für Mustererkennung und Bildverarbeitung, Institut für Informatik, Albert-Ludwigs-Universität Freiburg, Georges-Köhler-Allee, Freiburg, Germany;Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg, Jena, Germany;Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg, Jena, Germany;Institut für Physikalische Chemie, Friedrich-Schiller-Universität Jena, Helmholtzweg, Jena, Germany

  • Venue:
  • BioMed'06 Proceedings of the 24th IASTED international conference on Biomedical engineering
  • Year:
  • 2006

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Abstract

For the classification of biological samples based on Raman spectra, a robust classifier is necessary. This requirement is met by using Support Vector Machines (SVMs) enhanced by incorporating a-priori knowledge about pattern variations. In the described approach transformation knowledge is included directly into the classification process by using regularized tangent distance kernels. This approach replaces the standard Euclidean distance in the kernel function by the distance of the linear approximation (tangent spaces) of known transformation manifolds. These transformations represent first a global scaling of the spectral values referring to intensity variations, and second a baseline shift by Lagrange polynomials. Experiments are carried out and reported in this paper. The results show, that incorporating a-priori knowledge by tangent distances improves the classification rates substantially, while a lossy baseline correction becomes superfluous.